|
|
|
|
|||
|
|
|||||
|
|
|
|
|||
|
|
|||||
Cytological and histological structures identification with the technique IBIL in elemental microanalysis
|
Presented at 7th Int. Conf. on Nuclear Microprobe Technology and Applications, Bordeaux, (F), September 2000, and published on Nuclear Instruments and Methods in Physics Research B 181 (2001) 437-442 |
|
aP. Rossi, bC. Di Maggio, cG. P. Egeni, dA. Galligioni, aG. Gennaro, bL. Giacomelli, eA. Lo Giudice, aM. Pegoraro, bL. Pescarini, cV. Rudello and eE. Vittone |
|
a Department of Physics and INFN, via Marzolo 8, I-35131 Padua, Italy b Department of Oncology Sciences, Padua, Italy c Laboratori Nazionali di Legnaro, INFN, Legnaro(PD), Italy d Institute of Pathological Anatomy, Padua, Italy e Department of Experimental Physics and INFN, Turin, Italy |
|
Keywords: Nuclear microprobe; IBIL; Fluorescence; Biological cells; Tissues |
Abstract
Ion beam induced luminescence (IBIL) is applied to the inspection of histological and cytological specimens, dried and placed in vacuum. It is shown to offer a way for a precise aiming of a proton microbeam on the sample for a subsequent traditional microanalysis. We used the nuclear microprobe of the Laboratori Nazionali di Legnaro and its IBIL facility to identify biostructures stained with some usual fluorescent dyes, capable of discriminating tissues and cells of different nature or different parts of a cell. To this purpose we produced low dose IBIL maps of the region of interest, employing a high sensitivity light detector. We describe the experimental set-up, propose a peculiar support for specimens, specify the properties of few widespread used staining procedures and evaluate their IBIL emission.
